Journal: Human Cell

Article Title: Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2

doi: 10.1007/s13577-022-00844-9

Figure Lengend Snippet: PDCD4 knockdown induced p21 expression in LX-2 cells. a LX-2 cells were treated with negative control, p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The p21 mRNA levels were upregulated by PDCD4 knockdown in LX-2 cells. The data represent the average of three separate experiments. t test, * p < 0.05, ** p < 0.005. b A western blotting assay of PDCD4 knockdown LX-2 cells. LX-2 cells were treated with p2 (left panel) and k603 (right panel) PDCD4-specific siRNAs. Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in b . c p-p53 (Ser15) and p53 assay of PDCD4 knockdown LX-2 cells by western blotting. LX-2 cells were treated with p2 and k603 PDCD4-specific siRNAs. Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in c . d The relative amount of p21 protein was determined from b of LX-2 cells treated with p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The data represent the average of three separate experiments. t test, * p < 0.05. e The relative amount of CSL protein determined from b of LX-2 cells treated with p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The data represent the average of three independent experiments. t test, * p < 0.05. f The relative amount of p-p53 (Ser15) (upper panel) and p53 (lower panel) proteins determined from c of LX-2 cells treated with p2 and k603 PDCD4-specific siRNAs

Article Snippet: Antibodies against β-actin, Rb, phospho-Rb at Ser 780 (p-Rb[780]), phospho-p53 at Ser15 (p-p53[Ser15]), p53, p21, CDK2, CDK4, CDK6, cyclin D1, RBPJ-κ/CSL and HRP-linked secondary antibodies for mouse and rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Knockdown, Expressing, Negative Control, Western Blot

Journal: Human Cell

Article Title: Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2

doi: 10.1007/s13577-022-00844-9

Figure Lengend Snippet: CSL knockdown upregulated the p21 protein level in LX-2 cells. a LX-2 cells were treated with CSL-specific siRNA (CSL-1 and CSL-2). Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in a . b The relative amount of p21 protein was determined from a . c The relative amount of p53 was determined from a . The data represent the average of three independent experiments. t test, * p < 0.05

Article Snippet: Antibodies against β-actin, Rb, phospho-Rb at Ser 780 (p-Rb[780]), phospho-p53 at Ser15 (p-p53[Ser15]), p53, p21, CDK2, CDK4, CDK6, cyclin D1, RBPJ-κ/CSL and HRP-linked secondary antibodies for mouse and rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Knockdown, Western Blot

Journal: Human Cell

Article Title: Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2

doi: 10.1007/s13577-022-00844-9

Figure Lengend Snippet: PDCD4 knockdown induced p21 expression in LX-2 cells. a LX-2 cells were treated with negative control, p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The p21 mRNA levels were upregulated by PDCD4 knockdown in LX-2 cells. The data represent the average of three separate experiments. t test, * p < 0.05, ** p < 0.005. b A western blotting assay of PDCD4 knockdown LX-2 cells. LX-2 cells were treated with p2 (left panel) and k603 (right panel) PDCD4-specific siRNAs. Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in b . c p-p53 (Ser15) and p53 assay of PDCD4 knockdown LX-2 cells by western blotting. LX-2 cells were treated with p2 and k603 PDCD4-specific siRNAs. Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in c . d The relative amount of p21 protein was determined from b of LX-2 cells treated with p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The data represent the average of three separate experiments. t test, * p < 0.05. e The relative amount of CSL protein determined from b of LX-2 cells treated with p2 (upper panel) and k603 (lower panel) PDCD4-specific siRNAs. The data represent the average of three independent experiments. t test, * p < 0.05. f The relative amount of p-p53 (Ser15) (upper panel) and p53 (lower panel) proteins determined from c of LX-2 cells treated with p2 and k603 PDCD4-specific siRNAs

Article Snippet: Antibodies against β-actin, Rb, phospho-Rb at Ser 780 (p-Rb[780]), phospho-p53 at Ser15 (p-p53[Ser15]), p53, p21, CDK2, CDK4, CDK6, cyclin D1, RBPJ-κ/CSL and HRP-linked secondary antibodies for mouse and rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Knockdown, Expressing, Negative Control, Western Blot

Journal: Human Cell

Article Title: Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2

doi: 10.1007/s13577-022-00844-9

Figure Lengend Snippet: CSL knockdown upregulated the p21 protein level in LX-2 cells. a LX-2 cells were treated with CSL-specific siRNA (CSL-1 and CSL-2). Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in a . b The relative amount of p21 protein was determined from a . c The relative amount of p53 was determined from a . The data represent the average of three independent experiments. t test, * p < 0.05

Article Snippet: Antibodies against β-actin, Rb, phospho-Rb at Ser 780 (p-Rb[780]), phospho-p53 at Ser15 (p-p53[Ser15]), p53, p21, CDK2, CDK4, CDK6, cyclin D1, RBPJ-κ/CSL and HRP-linked secondary antibodies for mouse and rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Knockdown, Western Blot

Journal: Human Cell

Article Title: Induction of premature senescence and a less-fibrogenic phenotype by programmed cell death 4 knockdown in the human hepatic stellate cell line Lieming Xu-2

doi: 10.1007/s13577-022-00844-9

Figure Lengend Snippet: Expression of cell cycle regulatory factors in LX-2 cells. a LX-2 cells were treated with p2 PDCD4-specific siRNA (left panel) and k603 PDCD4-specific siRNA (right panel). Protein bands were analyzed by western blotting using antibodies against the respective proteins indicated in a . The data represented one of the three experiments. b The relative amount of Rb, p-Rb (780) (pRb), cyclin D1, CDK2, CDK4, and CDK6 proteins of p2-specific PDCD4 knockdown cells was determined from a (left panel). c The relative amount of Rb, p-Rb (780) (pRb), cyclin D1, CDK2, CDK4, and CDK6 proteins of k603-specific PDCD4 knockdown cells was determined from a (right panel). The data of b and c represent the average of three individual experiments. t test, * p < 0.05

Article Snippet: Antibodies against β-actin, Rb, phospho-Rb at Ser 780 (p-Rb[780]), phospho-p53 at Ser15 (p-p53[Ser15]), p53, p21, CDK2, CDK4, CDK6, cyclin D1, RBPJ-κ/CSL and HRP-linked secondary antibodies for mouse and rabbit antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA).

Techniques: Expressing, Western Blot, Knockdown